Preparation of anti-rat albumin antiserum and its application to radioimmunoassay.
نویسندگان
چکیده
Albumin was highly purified from a commercially available rat albumin preparation (Fraction V) using disc electrophoresis. The purified albumin had the same antigenicity as Fraction V. A monospecific anti-rat albumin rabbit serum was obtained. The antiserum was used in a double antibody quantitative method for determining rat albumin.
منابع مشابه
Biol. Pharm. Bull. 28(2) 340—343 (2005)
the treatment of congestive heart failure and atrial fibrillation. It is important to measure digoxin in serum from digitalized patients because the therapeutic range of serum digoxin concentration is narrow. Immunoassays are routinely used for therapeutic drug monitoring (TDM) of digoxin. The antibody with cross-reactivity to metabolites of digoxin (digoxigenin bis and monodigitoxosides, digox...
متن کاملRadioimmunoassay for metabolites of 9,3"-diacetylmidecamycin, a macrolide antibiotic.
A radioimmunoassay system has been developed for the measurement of two major metabolites of 9,3"-diacetylmidecamycin, Mb-6 and Mb-12. A radioimmunoassay for Mb-6 was performed by using anti-Mb-6 serum and a [125I]tyramined Mb-6 derivative as a radiolabeled antigen. The labeled antigen was prepared by the chloramine T method. The antiserum was obtained from a rabbit immunized with Mb-6 conjugat...
متن کاملDevelopment of radioimmunoassay for measurement of serum digoxin in digitalized patients using novel anti-digoxin antiserum.
There is an antiserum elicited by digoxin 3'-hemisuccinate-bovine serum albumin (BSA) conjugate possessing high specificity for digoxin. Our study focused on development of RIA using this novel antiserum for measurement of digoxin in serum from digitalized patients. The property of the new antiserum was investigated by RIA with digoxin 3'-hemisuccinyl-[3H]leucine. The separation of bound and fr...
متن کاملProduction and Characterization of Anti-LH Polyclonal Antibodies for Establishment of Sepharose Solid Phase Radioimmunoassay
The present study was designed to achieve the production and characterization of polyclonal antibody of luteinizing hormone (AntiLH) as a basic component of LH radioimmunoassay (RIA). The main objective was to improve the immunogenicity of LH by conjugation of LH with bovine serum albumin (LH: BSA) as a protein carrier using Ethyl dimethylaminopropyl Carbodiimide (ECDI). Production of Anti-LH w...
متن کاملTime-resolved fluoroimmunoassay for unconjugated estrogen in urine: comparison with a fluorometric assay for total estrogen and application in an in vitro fertilization program.
A time-resolved fluoroimmunoassay (TR-FIA) for unconjugated estrogens in human urine is described. 6-Keto-17 beta-estradiol-6-(O-carboxymethyl)oxime:bovine serum albumin is immobilized onto microtiter strip wells and the coated wells are incubated with 17 beta-estradiol standard preparations or unknowns with a polyclonal antiserum to 17 beta-estradiol-16,17-monosuccinyl:albumin. The antiserum-b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Acta medica Okayama
دوره 33 5 شماره
صفحات -
تاریخ انتشار 1979